Quaternary Ammonium Palmitoyl Glycol Chitosan (GCPQ) Loaded with Platinum-Based Anticancer Agents—A Novel Polymer Formulation for Anticancer Therapy

Quaternary ammonium palmitoyl glycol chitosan (GCPQ) has already shown beneficial drug delivery properties and been studied as a carrier for anticancer agents. Consequently, we synthesised cytotoxic platinum(IV) conjugates of cisplatin, carboplatin oxaliplatin by coupling via amide bonds to five GCPQ polymers differing in their degree palmitoylation quaternisation. The were characterised 1H 195Pt NMR spectroscopy well inductively coupled plasma mass spectrometry (ICP-MS), the latter determine amount units per polymer. Cytotoxicity was evaluated MTT assay three human cancer cell lines (A549, non-small-cell lung carcinoma; CH1/PA-1, ovarian teratocarcinoma; SW480, colon adenocarcinoma). All displayed high increase activity factors up 286 times compared corresponding complexes mostly outperformed respective platinum(II) counterparts 20 times, also taking into account loading Finally, biodistribution experiment performed with an oxaliplatin-based conjugate non-tumour-bearing BALB/c mice revealing increased accumulation tissue. These findings open promising opportunities further tumouricidal studies especially focusing on